Analysis Note

Positive ControlHCT116 or SW480 cells or colon tissue

Negative ControlLoVo cells

Application

Frozen Sections (5 µg/ml)r>Immunoblotting (1 µg/ml)r>Immunoprecipitation (1 µg/reaction)r>Paraffin Sections (3 µg/ml, heat pre-treatment required)

General description

Recognizes the ~100 kDa MSH2 protein in HCT116 and SW480 cells and colon tissue.

Purified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with SP20 mouse myeloma cells. Recognizes the ~100 kDa MSH2 protein.

This Anti-MSH2 (Ab-1) Mouse mAb (GB12) is validated for use in Frozen Sections, Immunoblotting, Immunoprecipitation, Paraffin Sections for the detection of MSH2 (Ab-1).

Immunogen

an N-terminal fragment of human MSH2

Human

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Bronner, C. E., et al. 1994. Nature368, 258.Papadopoulos, N., et al. 1994. Science263, 1625.Peltomäki, P. T. 1994. Annals of Medicine26, 215.Fishel, R., et al. 1993. Cell75, 1027-1038, 1993.Leach, F. S., et al. 1993. Cell75, 1215.Lindbolm, A., et al. 1993. Nature Genetics5, 279.

For paraffin sections, pre-treat with heat for 20 min. Antibody should be titrated for optimal results in individual systems.

Packaging

100 µg in Plastic ampoule

Please refer to vial label for lot-specific concentration.

Physical form

In 50 mM sodium phosphate buffer, 0.2% gelatin, pH 7.5.

Warning

Toxicity: Standard Handling (A)